Fig. 5

Relationship of relative abundance of the bacterial pilus with that relative abundance of E. coli, K. pneumonia, S. enterica, and C. nexile CAG:348, and its difference between the HC_LR and HC_HR groups. (A) Heatmap of relative abundance of the key discriminative species between the HC_LR and HC_HR groups and their contribution to bacterial pilus (GO: 0009289). Red text denotes species enriched in the HC_HR group. Correlation between pilus gene abundance and species relative abundance was examined using the Spearman correlation analysis. (B) Comparison of the relative abundance of the bacterial pilus (GO: 0009289) in the HC_HR and HC_LR groups. Comparison between the HC_LR and HC_HR groups was made using the Wilcoxon rank-sum test. (C - F) Comparison of the relative abundance of E. coli (C), K. pneumonia (D), S. enterica (E), and C. nexile CAG:348 (F) in the HC_HR and HC_LR groups. The bacterial abundance was positively associated with the abundance of the bacterial pilus (GO: 0009289). Comparisons between HR and LR groups and between the highest tier and other tiers were made using the Wilcoxon rank-sum test. HC, n = 52; HC_LR, n = 31; HC_HR, n = 21; ***P < 0.001; **P < 0.01; *P < 0.05.