Fig. 6

Activation of Hedgehog pathway rescues IQGAP3 knockdown-induced inhibition of lung cancer migration, invasion and stemness. (A-B) Representative images and quantified data from wound healing assays performed in H1299 cells after IQGAP3 siRNA and SAG treatment. Scale bar = 200 μm. (C-D) Representative images and quantified data illustrating Transwell migratory assays conducted in H1299 and A549 cells following IQGAP3 siRNA and SAG treatment. Scale bar = 50 μm. (E-F) Assessment of invasion ability in H1299 and A549 cells using Transwell invasive assays after IQGAP3 down-regulation and SAG addition. Scale bar = 50 μm. (G-H) Sphere formation capacity of lung cancer cells evaluated through sphere formation assays after silencing IQGAP3 and SAG treatment. Scale bar = 100 μm. Data represent mean ± SD from experiments conducted in triplicate. **P < 0.01, ***P < 0.001, ns not significant.