Fig. 2 | Scientific Reports

Fig. 2

From: Efficient identification of genomic insertions and surrounding regions in two transgenic maize events using third-generation single-molecule nanopore sequencing technology

Fig. 2

Southern blot detection of the bar gene in transgenic maize events ND4401 and ND4403. (A) M represents Trans15K DNA Marker; P represents positive plasmid (pCAMBIA1301-ZmNRT1.1 A-bar); 1 represents genomic DNA of T4 transgenic maize event ND4401 completely digested with Sac I; 2 represents genomic DNA of T5 transgenic maize event ND4401 completely digested with Sac I; 3 represents genomic DNA of T6 transgenic maize event ND4401 completely digested with Sac I; 4 represents WT. (B) M represents Trans15K DNA Marker; P represents positive plasmid (pCAMBIA1301-ZmNRT1.1 A-bar); 1 represents genomic DNA of T4 transgenic maize event ND4403 completely digested with Xma I; 2 represents genomic DNA of T5 transgenic maize event ND4403 completely digested with Xma I; 3 represents genomic DNA of T6 transgenic maize event ND4403 completely digested with Xma I; 4 represents WT. Note: T4, T5, and T6 represent the fourth, fifth, and sixth generations, respectively, of self-pollinated transgenic plants. WT refers to the wild-type maize line y822.

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