Fig. 2 | Scientific Reports

Fig. 2

From: A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis

Fig. 2

AC112721.1 promoted TNBC cell growth and migrationin vitro and in vivo. (A) The relative expression of AC112721.1 in four breast cancer cell lines and one immortalized human mammary cell line (MCF-10 A). (B) The expression efficiency of plasmid-mediated AC112721.1 knockdown was detected in MDA-MB-231 cell line using qRT-PCR. (C) CCK-8 assays showing the growth of MDA-MB-231 cells treated with sh-AC112721.1. (D) FACS was performed to determine the apoptosis rate of MDA-MB-231 cells after knockdown of AC112721.1. (E) Transwell assays in MDA-MB-231 cells treated with sh-AC112721.1. (F) The expression efficiency of plasmid-mediated AC112721.1 overexpression was detected in MDA-MB-231 cell line using qRT-PCR. (G) CCK-8 assays assessed proliferation of MDA-MB-231 cells transfected with pcDNA-AC112721.1. (H) Cell migration ability of MDA-MB-231 cells was determined after infection with pcDNA-AC112721.1. (I) Tumor volume was measured every 2 days. (J) Images of tumors of each group. K. Tumor weights of each group. NC negative control. FACS, fluorescence activated cell sorting. ANOVA was used for statistical difference analysis. All experiments were performed at least thrice with triplicate samples. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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