Fig. 4

Induction of IL-10 in B cells of FA patients is impaired. (A,B) CD19⁺CD45R⁺ naïve B cells were isolated from PBMCs of HC subjects (n = 10) and FA patients (n = 10), and exposed to LPS at indicated concentrations for two days. Bars show mean ± SD of IL10 mRNA levels in B cells (A) and IL-10 in culture supernatant (B). (C−E) naïve B cells were stimulated by LPS (10 µg/ml) or PBS for 48 h and analyzed by indicated procedures. Bars show mean ± SD of MAF mRNA (C), MAF (D) or Pol II (E) in IL10 promoter locus of B cells. Each dot in bars presents one sample. Statistics: ANOVA + Bonferroni test (A,B) or Student’s t-test (C–E). p values are presented in figures where appropriate. HC healthy control, FA food allergy, FA B cell B cell of FA patients, HC.BC B cells of HC subjects, FA.BC B cells of FA patients.