Fig. 3

The impacts of KRT7 on PDAC proliferation and metastasis in vitro. (A,B) Growth in the 3D tumor spheroid formation assay. A growth curve was generated by normalizing the tumor volume to the initial volume; n = 3 biologically independent samples; scale bar: 50 μm. (C) Western blot analysis of PCNA levels in PDAC and ASPC1 cells. (D) CCK-8 assay. (E,F) Migration of tumor cells in the 3D tumor spheroid formation assay. The migration area is expressed relative to the initial tumor spheroid area during spheroid migration. The quantitative data are shown on the right; n = 3 biologically independent samples; scale bar = 50 μm.