Fig. 2 | Scientific Reports

Fig. 2

From: The regulatory role of ACP5 in the diesel exhaust particle-induced AHR inflammatory signaling pathway in a human bronchial epithelial cell line

Fig. 2

DEP regulates apoptosis and ROS responses in ACP5 KO BEAS-2B cells. (A, B) Naïve and ACP5 KO BEAS-2B cells were treated with DEP (0–100 µg/mL) for 24 h. Apoptosis and necrosis rates were measured by labeling with Annexin-V-FITC and 7-AAD for flow cytometry analysis. Cells were stimulated with DEP (50 µg/mL) for 24 h. (C) Naïve and ACP5 KO BEAS-2B cells were incubated with DEP at the indicated concentrations for 6 h. Intracellular ROS levels were measured using DCFDA and analyzed by flow cytometry. (D) Naïve and ACP5 KO BEAS-2B cells were pretreated with 10 mM of NAC for 30 min and incubated with 50 µg/mL of DEP for 24 h. Apoptosis rates were measured by labeling with Annexin-V-FITC and 7-AAD for flow cytometry analysis. *p < 0.05, **p < 0.01, ***p < 0.001.

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