Fig. 1
Cytotoxic effect of DC-CIK cells on primary glioblastoma cell lines (84, G35 and BTSC233). (A) 84 cells were cultured for 24 h at different effector: target (E: T) ratios of 10:1, 20:1 and 40:1. (B) G35 cells were cultured for 24 h at different E: T ratios of 10:1, 20:1 and 40:1. (C) BTSC233 (as stem cells) were cultured for 24 h at different E: T ratios of 1:1, 5:1 and 10:1. (D) BTSC233 (as differentiated cells) were cultured for 24 h at different E: T ratios of 1:1, 5:1 and 10:1. The cytotoxic effect of DC-CIK cells was measured by flow cytometry. The results represent data from three separate experiments and are presented as mean ± SD. Significance levels were determined using two-way ANOVA with Bonferroni’s post-hoc test (*P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
