Fig. 4 | Scientific Reports

Fig. 4

From: MAP4 kinase-regulated reduced CLSTN1 expression in medulloblastoma is associated with increased invasiveness

Fig. 4

CLSTN1-mNG internalization and trafficking of CLSTN1-mNG. (A) Still image of CLSTN1-mNG overexpressing UW228 cell (Movie 1). An inverted greyscale of the green fluorescent signal is shown. Squares a – d depict different subcellular localization of CLSTN1-mNG. a: Lamellipodia-like structure at cell leading edge (Movie 2); b: Perinuclear near Golgi-like structure (Movie 3); c: in small vesicles at cell trailing edge; d: on filamentous structures connecting the cell core with the periphery (Movie 5. (B) Still image series of Movies 6 and 7 showing anterograde trafficking of CLSTN1-mNG in UW228 cell. Inverted greyscale images of the green fluorescent signals are shown. Red arrowheads indicate a filamentous structure progressing from the perinuclear region toward the cell periphery. Three passages of such structures are observed in the 10 min observation period. Red asterisks indicate the position of the front of filamentous structures at the indicated time point.

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