Fig. 4 | Scientific Reports

Fig. 4

From: Polysaccharide from Prinsepia utilis Royle maintains the skin barrier by mediating differentiation, lipid metabolism and tight junction of keratinocyte

Fig. 4

Norathyriol and PPARα shRNA lentivirus alleviate PUR-P-induced upregulation of PPARα expression. (A,B) Western blot (A) and qRT-PCR (B) assays characterized the effect of shRNA lentivirus on the expression of PPARα protein and mRNA in keratinocytes. (C,D) Representative images of PPARα gel blot (C) and PPARα mRNA expression in different groups (D). PUR-P facilitates the expression of PPARα protein and mRNA in keratinocytes, and this is limited by Norathyriol (PPARα antagonist) and PPARα shRNA lentivirus. The original gel blot is shown in the Supplementary Information file. N = 3; One-way ANOVA test with Tukey’s multiple comparisons test; Statistical significance was defined as P < 0.05.

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