Fig. 6

Reduced ZC3H12D expression inhibits lung tumor cell proliferation and invasion. A), Western blot comparison between the siRNA group and the negative control (NC) group. ZC3H12D and β-ACTIN were detected on separate gels. The siRNA knockdown group was performed in triplicate with similar results across the three repeats. The band closest to the NC group was cropped and shown as a representative result. Full-length gels for both ZC3H12D and β- ACTIN are provided in Fig. S5; B), Bar plot displaying the quantitative analysis of western blot data; C), Clonogenic assay results comparing the siRNA group with the NC group; D), Bar plot illustrating the quantification of cell colonies; E), Transwell assay results comparing the siRNA group with the NC group, scale bar indicates 100 μm (200 ×); F), Bar plot showing the quantification of invasive cells. *** indicates p < 0.0005; **** indicates p < 0.00005.