Fig. 5
CBS-H2S axis sustained the stability of MCM2 mRNA as well as the expression level of MCM2 by sulfhydrating RPS20. (A, B) Representative western blot images of MCM2, RPS20 and RPS20-ssh in colon epithelium cells of WT and CBS +/− mice. (C, D, E) Relative band intensity of MCM2, RPS20 and RPS20-ssh in mice. (F) Cell viability of Caco-2 WT cells and Caco-2 CBS KO cells with or without GYY4137. (G, H) Western blot of RPS20-ssh in Caco-2 WT cells and Caco-2 CBS KO cells with or without GYY4137 for 48 h. (I, J) Western blot analysis of RPS20, RPS20-ssh and MCM2 in Caco-2 cells after transfected plasmid. (K) Relative band intensity of RPS20, RPS20-ssh and MCM2. (L) ActD chase assays for the stability of MCM2 mRNA in Caco-2 cells treated with or without GYY4137. (a) Stability of MCM2 mRNA in WT Caco-2 cells. (b) Stability of MCM2 mRNA in RPS20C70A Caco-2 cells. (c) Stability of MCM2 mRNA in RPS20C36A Caco-2 cells. * P < 0.05, ** P < 0.01, *** < 0.001, **** < 0.0001, vs WT group. # P < 0.05, ## P < 0.01, vs CBS KO or ActD group.
