Fig. 4 | Scientific Reports

Fig. 4

From: Berberine treatment inhibits ferroptosis in NIT-1 murine pancreatic cell line via inhibiting OGT expression levels

Fig. 4

Overexpression of OGT reversed the berberine roles in the HG stimulated NIT-1 cells. The HG stimulated NIT-1 cells were treated with 0.4 µM dose of berberine and transfected with OGT overexpressed vector. (A) Then the cell viability was determined by CCK-8 assay. The ROS (B), Iron (C), MDA (D), GPX4 (E) and GSH (F) levels were detected by corresponding kits. (G) The cell death was detected by PI staining. (H) The protein levels of FTH1, NCOA4, DMTA, ACSL4, TFRC and SCL7A11 were detected by western blot. N = 3. *P < 0.05, **P < 0.01, ***P < 0.001.

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