Fig. 13

Analysis of caspase 3 in rat kidney using immunohistochemistry. There was no caspase 3 expression in the control group (negative) (A). (B) The group of rats with gentamicin-induced acute renal failure (ARF) whose renal tubular epithelial cells showed an elevated level of caspase-3 immunoreactivity in their cytoplasm. Caspases-3 are positive when the color is brown. There was no statistically significant increase in caspase-3 immunostaining in the groups treated with nanospanlastic (D) and CS (C). There was a smaller reduction in caspase-3 immunostaining in the groups treated with GSH and LS (E) and (F). The immunostaining for caspase-3 was not significantly reduced in the GSH-NPs and LS-NPs groups. Groups I and J, which are combination of GSH-LS and GSH-LS-NPs, exhibited a significant reduction in caspase-3 immunostaining.