Table 5 Tests of the robustness of an invented technique for GAB and MET.
Analyte | Chromatographic parameters | wavelength (nm) | Acetonitrile% ratio | Flow rate | |||
|---|---|---|---|---|---|---|---|
GAB | Â | 208 | 212 | 4.00% | 6.00% | 1.9 min/mL | 2.1 min/mL |
Assay % | 100.05% | 100.60% | 99.94% | 100.08% | 110.97% | 90.89% | |
Retention time (Rt) | 1.426 | 1.424 | 1.435 | 1.420 | 1.436 | 1.421 | |
Tailing factor | 1.00 | 1.00 | 1.03 | 1.03 | 1.04 | 1.04 | |
Resolution | N/A | N/A | N/A | N/A | N/A | N/A | |
MET | Assay % | 100.08% | 100.41% | 99.53% | 99.60% | 99.88% | 98.69% |
Retention time (Rt) | 5.377 | 5.367 | 5.386 | 5.366 | 5.386 | 5.365 | |
Tailing factor | 1.26 | 1.28 | 1.33 | 1.25 | 1.34 | 1.31 | |
Resolution | 11.80 | 11.81 | 11.83 | 11.82 | 11.82 | 11.80 | |
