Fig. 2 | Scientific Reports

Fig. 2

From: Transcription factor EB improves hypoxic pulmonary hypertension in fetal rats by suppressing NLRP3 inflammasome activation via induction of mitophagy

Fig. 2

Hypoxia activates mitophagy in pulmonary arterioles of fetal rats (A) Representative transmission electron microscopy images of pulmonary arteriole endothelial cells from control and hypoxia groups, showing mitochondrial morphology. Scale bars: 5 µm and 1 µm. Yellow arrows indicate the nucleus and nuclear membrane; Red arrows indicate mitochondria; Blue arrows indicate autophagosomes and autolysosomes. (B) Quantitative comparison of mitochondria and autophagosomes/lysosomes between the two groups (n = 5 per group). (C) Representative immunohistochemical staining of LC3 in lung tissues between the two groups. Scale bar: 50 µm. (D) Quantitative comparison of the average optical density of LC3 staining between the two groups (n = 5 per group). (E) Western blot analysis of mitophagy-related proteins in lung tissues between the two groups (n = 3). (F) Comparison of relative protein expression levels of mitophagy-related proteins between the two groups (n = 3). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

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