Fig. 3 | Scientific Reports

Fig. 3

From: Transcription factor EB improves hypoxic pulmonary hypertension in fetal rats by suppressing NLRP3 inflammasome activation via induction of mitophagy

Fig. 3

Hypoxia activates the NLRP3 inflammasome in pulmonary arterioles of fetal rats (A) Representative immunohistochemical staining of NLRP3 in lung tissues from control and hypoxia groups. Scale bar: 50 µm. Black arrows indicate pulmonary arterioles; Red arrows indicate bronchi. (B) Quantitative comparison of the average optical density of NLRP3 staining between the two groups (n = 5 per group). (C) Representative immunohistochemical staining of caspase-1 in lung tissues from the two groups. Scale bar: 50 µm. Black arrows indicate positive stained cells. (D) Quantitative comparison of the average optical density of caspase-1 staining between the two groups (n = 5 per group). (E) Western blot analysis of NLRP3 inflammasome-related proteins in lung tissues from the two groups. (F) Comparison of relative protein expression levels of NLRP3 inflammasome-related proteins between the two groups (n = 3). * P < 0.05; ** P < 0.01; *** P < 0.001.

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