Fig. 2
From: Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles
γ-H2AX after treatment with NPs. (A) γ-H2AX after treatment with NPs. A549 cells were treated with NPs (0.01 mg/mL) for various time points. H3 (CBB staining) was used as a standard for equal loading of proteins for SDS-PAGE; (B) γ-H2AX after treatment with Al2O3-NPs, CuO-NPs, ZnO-NPs or TiO2-NPs. γ-H2AX was determined using western blotting, in which the intensity of each band was extracted using ImageJ version 1.38. The extent of γ-H2AX in cells treated with Al2O3-NPs, CuO-NPs, ZnO-NPs or TiO2-NPs versus untreated control cells was calculated. All the above blots had already been cropped in order to improve the clarity and conciseness of the presentation. The original blots are presented in Supplementary Fig. 7.
