Fig. 2
The inflammatory response of ECs is mediated by epithelial-derived CXCL10. (A) Calu-3 cells were infected with SARS-CoV-2 (MOI = 1) for 4 h and plated at the bottom of the transwell chamber before they were cocultured with vesseloids placed in the upper chamber for 3 days. (B) Relative VCAM-1 mRNA expression in vesseloids infected with SARS-CoV-2 alone or after coculture with Calu-3 cells infected with SARS-CoV-2. Analyze was performed 3 days post infection (MOI = 0.1). The values were normalized according to tubulin mRNA expression. The results are presented as the means ± SEMs (unpaired t test; *P < 0.05). (C) Heatmap of pixel density quantification from the cytokine array assay for selected chemokines and cytokines from vesseloids infected alone or after coculture with Calu-3 cells. SARS-CoV-2 was added to the culture supernatant, which was incubated for 4 h and analyzed 3 days post infection (MOI = 1). (D) Relative CXCL10 mRNA expression in SARS-CoV-2-infected vesseloids alone or after coculture with infected Calu-3 cells or infected Calu-3 cells alone. The data were analyzed 3 days post infection by SARS-CoV-2 (MOI = 0.1). The results are presented as the means ± SEMs (unpaired t test *P < 0.05). (E) CXCL10 concentration in the supernatant of infected vesseloids alone or after coculture with infected Calu-3 cells or infected Calu-3 cells alone. Analyze of the cells was performed 3 days post infection (MOI = 0.1). The results are presented as the means ± SEMs (one-way ANOVA, *P < 0.05).
