Fig. 3
Epithelial-derived CXCL10 opens inter-endothelial junctions. (A) HUVECs were seeded in the upper well of a transwell chamber and grown to confluency. Cells were treated with 1 µg/ml CXCL10 or 1 µg/ml VEGF-A for 1 h after 25 µg/ml FITC-dextran was added to the upper well. Every 20 min, after the addition of dextran, the fluorescence in the lower compartments was measured. The results are presented as the means ± SEMs (one-way ANOVA, *P < 0.05). (B) For quantification of VE-cadherin junctions, HUVECs were seeded, grown to confluency and treated for 4 h with 1 µg/ml CXCL10 or 1 µg/ml VEGF-A and VE-cadherin, and actin was stained. The results are presented as the mean ± SEM (one-way ANOVA, **P < 0.01). (C) VE-cadherin immunostaining after stimulation for 4 h with 1 µg/ml CXCL10 or 1 µg/ml VEGF-A. Green indicates VE-cadherin, red indicates actin, and blue indicates DAPI.
