Fig. 2

Havcr1 (Kim1) is increased in the cortex of both early and late-exposed rabbits compared to saline controls, however injury is greater in those exposed early. A Kim1 qPCR fold change in nephrotoxin-exposed animals relative to age-matched control samples and normalized by changes in beta-actin levels. B Quantification of Kim1 in cortex of RNAScope demonstrates increased Kim1 in nephrotoxin-induced samples, with significantly increased Kim1 in early-exposed rabbits compared to controls. C Kim1 expression is localized to the cortico-medullary border/inner cortex in early-exposed rabbits while sparing the nephrogenic zone. D Late-exposed rabbits still favor injury in the inner cortex although Kim1 staining is more diffuse.