Fig. 4 | Scientific Reports

Fig. 4

From: Metformin inhibits the growth of SCLC cells by inducing autophagy and apoptosis via the suppression of EGFR and AKT signalling

Fig. 4

Metformin activates autophagy in SCLC cells. (A) Autophagy-related proteins, including LC3, P62, and Beclin1, were detected by western blotting after H446, H446/DDP, H526, and H526/DDP cells were treated with different concentrations of metformin (0, 5, 10, and 20 mM) for 48 h. (B) The protein levels of p62 were detected by western blotting after the cells were treated with 10 mM metformin for 12, 24, 36, or 48 h. (C) Immunofluorescence analysis of LC3 in H446 and H446/DDP cells treated with or without metformin for 48 h. The LC3 protein was labelled green (Alexa 488), and the nuclei were labelled blue (DAPI). (D) The expression of LC3 and p62 was examined in H446 and H446/DDP cells after treatment with 3-MA (5 mM) combined with metformin (10 mM) for 48 h. Significant difference compared with the untreated control: *p < 0.05; **p < 0.01; ***p < 0.001. n = 3.

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