Fig. 4

Impact of BZYQD on the proliferation and migration of HCT116 cells in vitro. (A, B) HCT116 cells were exposed to a blend of BZYQD at concentrations of 0, 100, 200, 400, 800, and 1600 µg/ml. An MTT assay was conducted to evaluate cell viability, and IC50 values were determined. Additionally, HCT116 cells were treated with the primary components of BZYQD at concentrations of 165, 330, and 660 µg/ml. (C, D) Cell proliferation was assessed using EdU labeling, with DNA stained blue and proliferating cells stained green. (E) The migratory capacity of HCT116 cells was analyzed through a wound healing assay. (F) A Transwell migration assay was also employed to assess the migration ability of HCT116 cells. The data are expressed as mean ± SEM (n = 3). *P < 0.05 and **P < 0.01 in comparison to the control group.