Fig. 2

MIR-340-5p and miR-424-5p directly target the PD-L1. Following the transfection of SNK6 and SNK6R cells with miR-340-5p and miR-424-5p inhibitors or mimics, the expression levels of PD-L1 protein (A and B) and mRNA (C) were determined by western blot and RT-qPCR, respectively. (D) After overexpression of miR-340-5p or miR-424-5p, the enrichment of PD-L1 mRNA was examined using Ago2 IP, assessed by RT-PCR. (E and F) The schematic diagram showed the binding sites of miR-340-5p and miR-424-5p on the 3′UTR of PD-L1. Luciferase reporter assays were conducted on cells co-transfected with a reporter construct containing either the wild-type (WT) or mutant (MU) 3′UTR of PD-L1 and the miR-340-5p and miR-424-5p mimics or mimic-NC. Luciferase expression was measured using a Dual-GLO™ Luciferase Assay System. Data represent mean ± SEM. All experiments were repeated three times.