Fig. 4 | Scientific Reports

Fig. 4

From: Starvation-induced HBP metabolic reprogramming and STAM2 O-GlcNAcylation facilitate bladder cancer metastasis

Fig. 4

STAM2 S375 O-GlcNAcylation enhances its stability by decreasing ubiquitination. Half-life and quantitative analysis of STAM2-HA (A-B) and STAM2 S375A-HA (C-D) in UM-UC-3 cells. E Co-IP assays detected the interaction between STAM2-HA and Ub-Flag in UM-UC-3 cells transfected with STAM2-HA. (F) Quantitative western blot analysis of ubiquitination. (G) Co-IP assays detected the interaction between STAM2-HA and Ub-Flag in UM-UC-3 cells transfected with STAM2 S375A-HA. (H) Quantitative western blot analysis of ubiquitination. (I) Immunofluorescence staining of STAM2-HA and OGT-Flag in UM-UC-3 cells treated with OSMI-1 or PUGNAc. (J) Quantification of the nuclear/cytoplasmic fluorescence ratios of STAM2. (All PVDF bands were transferred and tested in the same western blot experiments, and the experiments performed in triplicate.)

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