Fig. 5
STAM2 S375 O-GlcNAcylation facilitates the JAK2–STAT3 signaling pathway. (A) STAM2 protein levels were measured using western blotting. (B) JAK2/p-JAK2 and STAT3/p-STAT3 protein levels were measured using western blotting in NC cells, STAM2-overexpressing cells and STAM2-knockout cells. (C) Quantitative western blot analysis of B. (D) JAK2/p-JAK2 and STAT3/p-STAT3 protein levels were measured using western blot in UM-UC-3 cells transfected with STAM2-HA or STAM2 S375-HA and treated with PUGNAc or not. (E) Quantitative western blot analysis of D. (F-G) Proliferation, migration, and invasion were measured using CCK-8 and Transwell assays in NC cells or cells transfected with STAM2-HA or STAM2 S375-HA after treatment with JSI-124. (H) EMT marker protein levels were measured using western blotting. (I) Quantitative western blot analysis of H. All PVDF bands were transferred and tested in the same western blot experiments. All the results represent three distinct repetitions. Statistical significance is denoted as ***p < 0.001, **p < 0.01, and *p < 0.05, indicating significant differences between two groups.
