Fig. 6
STAM2 S375 O-GlcNAcylation promotes tumor proliferation and metastasis in vivo. (A) Schematic representation of the establishment of UM-UC-3-derived xenografts. (B) Representative images of xenograft tumors 21 days after subcutaneous injection. C Tumor volumes were measured every 3 days. (D) Weights of xenograft tumors 21 days after subcutaneous injection. (E-H) Protein levels of EMT marker JAK2/p-JAK2 and STAT3/p-STAT3 were measured using western blotting. All PVDF bands were transferred and tested in the same western blot experiments, and the experiments performed in triplicate. (I-L) Bioluminescence detection and bioluminescence quantitative analysis of the lung (I-J) and popliteal lymph nodes (K-L). (M-N) Representative image of the popliteal lymphatic metastasis model, along with quantitative analysis of the volume of popliteal lymph nodes.
