Fig. 7

Upregulation of AC133552.2 significantly suppresses osteosarcoma cell proliferation and migration while promoting apoptosis. (a,c) AC133552.2 expression was significantly increased in the 143B and U2OS cell lines. (b,d,e,f) CCK-8 and EdU assays demonstrated that upregulation of AC133552.2 significantly inhibited the proliferation of 143B and U2OS cells. (g,h) Transwell assays indicated a significant decrease in the migration capacity of 143B and U2OS cells following AC133552.2 upregulation. (i,j) Scratch assays further confirmed that the migration ability of 143B and U2OS cells was significantly reduced after upregulation of AC133552.2. (k,l) Apoptosis assays revealed a significant increase in the apoptosis rate of MG63 and U2OS cells following the upregulation of AC133552.2. The data were presented as the mean ± SD with n = 3. ***p < 0.001, **p < 0.01, *p < 0.05.