Table 3 Nucleotide polymorphisms determined in a whole genome of an ASFV-G-∆MGF vaccine-like variant (VN/sows_32005/23) compared to a high-virulent Georgia 2007/1 isolate and masterseed strain.

1363

5ʹ UTR

–

A

A

No effect

1384

Non-coding

–

CC

CC

No effect

11,839

MGF110-8 L

C

C

T

Val ◊ Met

14,228

MGF110-1 L–MGF110-14 L fusion (Poly C region)

CCC

CC-

–

Delete 1 aa

15,666

MGF110-13Lb (Poly C region)

C

–

–

Reading frame shift

17,624

Non-coding

–

GG

G-

No effect

17,838

Non-coding

–

G

G

No effect

19,793

Non-coding

G

–

–

No effect

20,009

ASFV G ACD 00350 (Poly G region)

(G)16

(G)15

(G)10

Delete 2 aa

21,797

Non-coding

–

GG

G-

No effect

27,420

Non-coding

T

–

–

No effect

36,932

MGF505-4R

T

T

C

Silent mutation

36,938

T

T

C

74,823

EP402R

–

–

AATAT

Reading frame shift

81,759

M448R

T

C

T

His ◊ Tyr^***

98,396

B438L

A

G

G

Silent mutation

98,589

B169L

A

A

G

Silent mutation

145,579

D205R

G

G

A

Gly ◊ Arg

145,583

G

G

A

Gly ◊ Asp

167,189

E199L

C

G

G

Ala ◊ Pro

170,454

Non-coding

TG

–

TG

No effect

185,175

DP71L

C

C

A

Val ◊ Leu