Fig. 3

Effects of SOCS6 on the inflammatory and oxidative state of NRK cells. (A) The relative expression of SOCS6 in NRK cells transfected with miR-203 mimic or inhibitor was detected by RT-PCR. (B) The proliferation of NRK cells overexpressed or deficient with SOCS6 was measured by CCK8 assay. (C) The concentrations of TNF-α, IL-1β, IL-4, and IL-6 in NRK cells overexpressed or deficient with SOCS6 were detected by ELISA. (D, E) The levels of ROS (D) and MDA (E) in NRK cells overexpressed or deficient with SOCS6 were detected by assay kits. N = 3. **P <0.01, ***P < 0.001, ****P < 0.0001, compared with LG group; ^# P < 0.05, ^## P < 0.01, ^### P < 0.001, ^#### P < 0.0001, compared with HG group.