Fig. 5
From: MiR- 150 deletion promotes lung tumor growth by upregulating P-STAT3 and ROS in MDSCs
The disappearance of miR- 150 augmented the expression of IRE1α and P-STAT3 proteins in MDSCs. (A-B)qRT-PCR was used to analyze BM-derived MDSC transfected with miR- 150 mimics or inhibitors. (C) Western blot was used to detect the expression of IRE1a and P-STAT3 in BM-derived MDSCs transfected with miR- 150 mimics or inhibitors. (D-E) Statistical analysis of IRE1α or P-STAT3. (F) qRT-PCR validated the lower expression of miR- 150 in BM-derived MDSCs of miR- 150 KO. (G) Protein analysis of IRE1α and P-STAT3 in BM-derived MDSC from WT and miR- 150 KO mice. (H) Analysis of IRE1α or P-STAT3 in BM-derived MDSC from WT and miR- 150 KO mice. *P < 0.05, **P < 0.01, ***P < 0.001; ns, no significance.
