Fig. 4
From: TIRE-seq simplifies transcriptomics via integrated RNA capture and library preparation
Tracking Temporal and Dose-Dependent Changes in patient-derived neurospheres with TIRE-seq. (a) Experimental design for TMZ-treatment of PDNs. Cells were exposed to varying doses of TMZ and sampled at three timepoints for TIRE-seq. A parallel culture was monitored for cell growth and death using IncuCyte imaging. (b) Growth curve (left) and caspase reactivity (right) from IncuCyte imaging. (c) Principal component analysis (PCA) visualization of the PDNs experiment. Samples are colored by timepoint and labeled by treatment: D = DMSO, T = Temozolomide. (d) LogCPM values of the top six most variable genes across timepoints across escalating TMZ doses. (e) Volcano plot of differentially expressed genes between 10Â \(\mu\)M TMZ and DMSO-treated neurospheres following three days of treatment. (f) Gene set enrichment analysis (GSEA) with hallmark gene sets comparing 10Â \(\mu\)M TMZ and DMSO-treated PDNs after three days of treatment.
