Fig. 7
TF-hub gene-miRNA network, drug prediction and qRT-PCR validation of hub genes. (A) TF-hub gene network (red V-shapes: hub genes, purple ellipses: TFs). (B) Hub gene- miRNA network (red ellipses: hub genes, green ellipses: miRNAs). (C) Integrated TF-hub gene-miRNA network (red V-shapes: hub genes, purple ellipses: TFs, green triangles: miRNAs). (D) The top 10 candidate drugs identified by 61 hub genes. The color gradient from brighter to darker indicates the significance of each drug (high to low). (E) Genes from Gene sets 1–4 subjected to further validation using qRT-PCR. (F) qRT-PCR validation of 11 genes (from panel (E)) in neonatal mice after SCI. (G) qRT-PCR validation of 11 genes (from panel (E)) in adult mice after SCI. Data are expressed as means ± standard errors of the mean (SEMs) (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001.
