Fig. 5
PE potentiates glutamate release at mPFC and LHb inputs onto DRNCeA 5-HT neurons. (A) Top panel is a schematic of viral injections of rAAV-CAG-tdTomato and AAV-Syn-ChR2 injections in the CeA and mPFC, respectively. Bottom panel depicts oEPSC traces induced by photostimulation of mPFC axon fibers in the DRN in the absence of TTX (red), in TTX (black), and in TTX + AP-5 (blue). (B) Scatter plots of the oEPSC amplitude evoked by photostimulation of mPFC fibers and recorded from DRNCeA neurons of PC (49.21 ± 2.76 pA, n = 22) and PE (49.27 ± 9.46 pA, n = 31, p = 0.99 vs. PC) rats. (C) Representative oEPSCs traces evoked by paired optical stimuli (blue bars) in PC and PE rats, scale bars: 50 ms and 25 pA. (D) Summary plot of paired-pulse ratio of oEPSCs recorded in PC (0.73 ± 0.031, n = 8) and PE (0.51 ± 0.056, n = 11, p = 0.006 vs. PC at 50 ms SI) rats. (E) Top panel, schematic of viral injections of rAAV-CAG-tdTomato and AAV-Syn-ChR2 in CeA and LHb, respectively. Bottom panel, representative sample oEPSC traces induced by photostimulation of LHb fibers recorded from DRNCeA neurons under baseline condition (orange), in TTX (black), and in TTX + AP-5 (blue). (F) Scatter plots of the amplitude of oEPSCs recorded from DRNCeA neurons in PC (50.74 ± 11.24 pA, n = 20) and PE (84.45 ± 15.13 pA, n = 37, p = 0.13 vs. PC) rats. (G) Representative oEPSCs traces evoked by a pair of optical stimuli in PC and PE rats, scale bars: 50 ms and 50 pA. (H) Summary plot of paired-pulse ratio of oEPSCs recorded in PC (0.99 ± 0.13, n = 9) and PE (0.33 ± 0.06, n = 6, p = 0.001 vs. PC at 50 ms SI) rats. Data are represented as mean ± SEM. *p < 0.05, n.s. not significant.
