Fig. 6
PE increases tonic NO signaling at LHb glutamate synapses of DRNCeA 5-HT neurons. (A) Schematic of rAAV-CAG-tdTomato injections in the CeA. (B1,B2) Immunostaining of tdTomato-labeled DRNmPFC neurons (red) and nNOS expression (green). (C) Quantification of tdTomato and nNOS expression in DRNCeA neurons. (D) Schematic of the ChR2 assisted analysis of mPFC -DRN-CeA neuronal circuit. (E1) Summary graph of the effect of nNOS activator SNAP on the amplitude of oEPSCs induced by photostimulation of mPFC fibers and recorded from DRNCeA neurons in PC (Baseline: 100.00 ± 0.23%, n = 5; SNAP: 129.26 ± 8.03%, n = 5, p = 0.043 vs. baseline) and PE (Baseline: 100.00 ± 0.30%, n = 6; SNAP: 121.04 ± 9.86%, n = 6, p = 0.16 vs. baseline) rats. (E2) Representative sample of oEPSCs traces taken before (1) and during SNAP application (2) in PC and PE rats. Scale bars: 25 ms, 25 pA. (F) Schematic of ChR2 assisted analysis of LHb -DRN-CeA neuronal circuit. (G1) Summary of the effect of nNOS activator SNAP (100 µM) on the amplitude of oEPSCs induced by photoactivation of LHb fibers and recorded from DRNCeA neurons in PC (Baseline: 100.00 ± 4.21%, n = 6; SNAP: 127.09 ± 6.79%, n = 6, p = 0.019 vs. baseline) and PE (Baseline: 99.05 ± 0.94%, n = 7; SNAP: 94.47 ± 8.50%, n = 7, p = 1 vs. baseline) rats. (G2) Representative sample oEPSCs traces taken before (1) and during SNAP application (2) in PC and PE rats. Scale bars: 50 ms, 50 pA.
