Fig. 2
From: Attenuated PINK1 autophosphorylation play neuroprotective and anti-seizure roles in neonatal hypoxia
Hypoxia-induced seizures were accompanied by apoptosis, mitophagy and neuronal damage. (A-E) The levels of active caspase-3, caspase-3 and P62 in the cortex and hippocampus were detected by western blotting. (F-H) The immunofluorescence intensity of LC3B (red) and TOMM20 (green) increased after hypoxia treatment (3 day; n = 4/group). DAPI, blue. Bar = 20 μm. (I-J) The neuronal damage of DG, CA3 and EC after hypoxia treatment detected by FJB staining (3 day; n = 4/group). Bar = 50 μm. Compared with controls, *P < 0.05, **P < 0.01, and ***P < 0.001 (one-way ANOVA). Mean ± SEM are shown. C cortex, H hippocampus, CA3 cornu ammonis area 3, DG dentate gyrus, EC entorhinal cortex, Hy Hypoxia, LC3B microtubule-associated protein light chain 3B, TOMM20 translocase of outer mitochondrial membrane 20.
