Fig. 3
From: Attenuated PINK1 autophosphorylation play neuroprotective and anti-seizure roles in neonatal hypoxia
Inhibition of TOM7 expression reduced the levels of phosphorylated PINK1/Parkin/Ub during hypoxia-induced seizures. (A,B) TOM7 (green) immunofluorescence intensity (3 day; n = 4/group). Bar = 50 μm. (C-H) Changed levels of PINK1/P-PINK1 and Parkin/P-Parkin due to TOM7 intervention (n = 4/group). (I-J) P-Ub (red) immunofluorescence intensity in the EC and DG (3 day; n = 4/group). DAPI, blue. Bar = 50 μm. Mean ± SEM are shown. Compared with controls, *P < 0.05, **P < 0.01, and ***P < 0.001 (one-way ANOVA). ###P < 0.001 compared with each other (one-way ANOVA with Dunnett’s T3 post-hoc test). C cortex, DG dentate gyrus, EC entorhinal cortex, H hippocampus, Hy Hypoxia, P-Parkin phosphorylated Parkin, P-PINK1 phosphorylated PTEN-induced kinase 1, P-Ub phosphorylated ubiquitin, TOM7 translocase outer mitochondrial membrane 7.
