Fig. 1

Screening of a natural compound library. HMVP2 cell viability following treatment with a library of natural compounds was screened using the ATP bioluminescence assay. HMVP2 cells were treated for 12 (blue), 24 (red) and 48 h (green) with a library of natural compounds (single agent) at the dose of 5, 10 or 20 µM (only the latter dose is shown in the figure). Natural compounds are sorted according to increasing ATP bioluminescence values of the 12 h time-point (list of compounds is included in Supplementary Table 1). Relative ATP levels (ATP level in treated samples normalized by ATP level of untreated samples) as averages of four replicates are shown