Fig. 5

Treatment-induced changes in HMVP2 intracellular glutamine flux. HMVP2 cells were treated with CUR, UA, RES or their combinations of two compounds and concurrently cultured in medium containing 13C5,15N2-glutamine (glutamine in the growth medium completely replaced with isotopically labeled glutamine). Schematic representation of the flux of isotopically labeled glutamine through the TCA cycle, and the aspartate and proline metabolic pathways. UHPLC-MS data were acquired and metabolite enrichment is shown as the fraction of enrichment of the total metabolite pool (average of three replicates)