Fig. 1: IKKα physically associates with DARPP-32 isoforms.

a-c Human lung adenocarcinoma cell lines HCC827 (a), PC9 (b), and H1975 (c) stably overexpressing FLAG-tagged human DARPP-32 isoforms were lysed and subjected to immunoprecipitation using anti-IKKα antibodies. Immunoprecipitated lysates were separated in SDS-PAGE and immunoblotted with antibodies against IKKα, FLAG (that detects exogenously overexpressed DARPP-32), and α-tubulin (loading control).