Fig. 7: Depletion of IKKα inhibits lung tumor cell growth and proliferation in vivo.

a Luciferase-labeled IKKα-depleted human HCC827 cells were orthotopically injected into the left thorax of SCID mice and imaged for luminescence on the indicated days. Total luminescence intensity (photon count) was calculated using molecular imaging software and plotted as a line graph. Error bars are shown as dotted lines indicating SEM. A value of P ≤ 0.05 was considered significant, two-way ANOVA followed by Sidak’s test. b Images of anesthetized mice were captured to detect luminescence signals on the indicated days. c Tumor tissue lysates obtained from either LacZ shRNA- or IKKα shRNA-transduced human HCC827 cells-derived xenografts were subjected to immunoblotting using primary antibodies against IKKα, phosphorylated PP1α (Thr320), total PP1α, phosphorylated ERK (Thr202/Tyr204), total ERK, and α-tubulin (loading control). Bar graphs show densitometric quantification values of the results from three western blotting experiments. *P < 0.05; Two-tailed unpaired t-test. d Overall morphological evaluation was performed on formalin‐fixed, paraffin‐embedded lung tissues (n = 5 mice per group) obtained from human HCC827 cells-derived lung tumor xenograft mice model using hematoxylin and eosin (H&E) dye. Scale bar 50 µm.