Fig. 3: Effect of Rab27A on exosome secretion in NSCLC cells. | npj Precision Oncology

Fig. 3: Effect of Rab27A on exosome secretion in NSCLC cells.

From: Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis

Fig. 3

a Functional enrichment analysis of differential protein showed that knockdown of Rab27A affects protein transport and protein localization function of NSCLC cells. b Transmission electron microscopy (TEM) image showed the appearance of exosomes isolated from NSCLC cell supernatant. c Nanoparticle tracking analysis (NTA) characterized the particle size distribution of isolated exosomes. d Western blot showed that the exosome markers TSG101, CD9, and CD81 in cell supernatant decreased significantly after the knockdown of Rab27A. GM130 and Calnexin were used as negative marker for exosome detection. e Conditioned medium (CM) collected from Rab27A-overexpression cells promoted the clonogenic ability of H1299 cells. f Conditioned medium (CM) collected from Rab27A-overexpression cells promoted the migration and invasion ability of A549 cells (Scale bar, 200 μm). g Conditioned medium (CM) collected from Rab27A-overexpression cells promoted the migration and invasion ability of H1299 cells (Scale bar, 200 μm). *P < 0.05; **P < 0.01; ***P < 0.001.

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