Fig. 8: STAT1 transcriptionally regulates HSPA5 gene in LUADs. | npj Precision Oncology

Fig. 8: STAT1 transcriptionally regulates HSPA5 gene in LUADs.

From: Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis

Fig. 8

a qRT-PCR analysis of HSPA5 mRNA in A549 and H1299 cells treated with IFNα at the indicated time. b, c Cell lysates from A549 and H1299 cells treated with Cerdulatinib and IFNα were subjected to Western blot analysis to detect the expression of HSPA5. d, e qRT-PCR analysis of STAT1 mRNA in A549 and H1299 cells with Rab27A-overexpression after STAT1-silencing. f, g qRT-PCR analysis of STAT2 mRNA in A549 and H1299 cells with Rab27A-overexpression after STAT2-silencing. h A conserved consensus HSPA5 response element was present. i Schematic drawing shows a predicted SBE in HSPA5 mRNA 3’UTR. Boxed areas indicate the HSPA5 3’UTR segment (position, −909 to −900), containing wild type (HSPA5-WT) or mutant of SBE (HSPA5-MUT). Numbers present the nucleotide position of HSPA5 mRNA. j Relative luciferase activities were determined as described in the “Methods” section and normalized to those in vector cells.

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