Fig. 9: Immunohistochemistry (IHC) staining was used to detect immune cells in the tumor. | npj Precision Oncology

Fig. 9: Immunohistochemistry (IHC) staining was used to detect immune cells in the tumor.

From: Integrating bulk, single-cell, and spatial transcriptomics to identify and functionally validate novel targets to enhance immunotherapy in NSCLC

Fig. 9

Immunohistochemical (IHC) analysis of CD8, CD4, and FOXP3 expression in syngeneic mouse lung cancer tumor tissues following Ptges3 silencing and Ctsh overexpression accompanying PD-1 antibody treatment. a–d Representative IHC staining images and quantitative analysis demonstrated that silencingPtges3 and overexpressing Ctsh significantly increased CD8+ and CD4+ T-cell infiltration while decreasing FOXP3+ regulatory T cells compared to the control group. These effects were further amplified in the PD-1 antibody-treated group, indicating enhanced antitumor immunity. Data are presented as mean ± SD, with statistical significance indicated. *P < 0.05, **P < 0.01, ***P < 0.001.

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