Fig. 2: The observed cryo-EM structures of six reaction states.
From: Coupled catalytic states and the role of metal coordination in Cas9
a, Electron potential density maps corresponding to the pre-cleavage (A), cleavage intermediates (B1 and B2), post-cleavage (C1 and C2) and target-bound (D) states. RuvC, RuvC nuclease ___domain. b, Cartoon representations of the structural models corresponding to the maps in a, where each ___domain or nucleic acid molecule is labelled in the same colour. c, Close-up views of the cleavage site of the TS DNA overlaid with the density for each of the corresponding states shown in a and b. The arrow indicates the position of the scissile phosphate bond.
