Extended Data Fig. 9: Flow cytometry of adipose and circulating immune cells from cont ASO and RIPK1 ASO treated mice. | Nature Metabolism

Extended Data Fig. 9: Flow cytometry of adipose and circulating immune cells from cont ASO and RIPK1 ASO treated mice.

From: RIPK1 gene variants associate with obesity in humans and can be therapeutically silenced to reduce obesity in mice

Extended Data Fig. 9

a, Representative flow cytometry plots and NKT % (expressed as % of F4/80- cells that are αGalCer-loaded CD1d-tetramer+CD3e+) in spleen from mice treated for 5 weeks with cont ASO or RIPK1 ASO (n=8/group). Data representative of mean ± SEM of n=8 mice/group, analyzed by two-tailed Student’s t-test. b, c, Flow cytometry analysis of circulating leukocytes from mice treated with control ASO or RIP1 ASO-B at 5 weeks: Ly6Clow monocytes *p<0.05 (p=0.0452) Ly6Chigh monocytes *p<0.05 (p=0.0108), and CD3e+ T cells. (c) Flow cytometry analysis of circulating leukocytes from mice treated with control ASO or RIP1 ASO-B at 22 weeks: Ly6Clow monocytes, Ly6Chigh monocytes and CD3e+ T cells (p=0.0132). Data in b, c representative of mean ± SEM of n=8 mice/group, analyzed by two-tailed Student’s t-test.

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