Extended Data Fig. 2: Single nuclei sequencing of human adipose tissue and HMDP data from mice.

a, Single-nuclei RNA sequencing on human adipose tissue: UMAP visual representation of 10 cell types derived from single-nucleus RNA-seq of human abdominal subcutaneous adipose tissue. Clusters are identified using the Seurat pipeline and cell types are assigned by biological function of marker genes. Vertical bar plot of average cell type-specific expression of RIPK1 across the 10 cell types identified in human abdominal subcutaneous adipose tissue. Expression was normalized using SCTransform. b, Data from the Hybrid Mouse Diversity Panel (HMDP) showing correlations of Ripk1 mRNA expression and NMR, visceral fat mass at sacrifice, serum insulin and HOMA-IR index in males from approximately 100 inbred strains of mice¹⁹. c, Luciferase reporter activity containing the variant sequence plus the human RIPK1 promoter upstream of the firefly luciferase gene (as in Fig. 2b). Data is shown as mean ± SEM of n=4 independent experiments, d–f. Single cell RNA sequencing of mouse CD45+ immune cells (from GSE128518) after 6, 12 and 18 weeks high fat diet feeding. d, Horizontal bar plot of cell clusters in normal chow (NC) and high fat diet (HFD). e, UMAP plot of Xcl1 cluster (cluster 9, representing NK/NKT cells) and (f) the corresponding expression of Nfil3 and Ripk1 in cluster 9.