Extended Data Fig. 5: GAS5 regulates FH-MDH2-CS association by declining MDH2 acetylation.
From: Mitochondrial long non-coding RNA GAS5 tunes TCA metabolism in response to nutrient stress
(a) The 3D molecular model of MDH2 tetramer was established using PyMol software and the indicated acetylation sites were marked in red. (b) S-protein beads pulldown coupled IB detection of SFB-MDH2 acetylation level in HEK293T, under normal (25 mM) or high (50 mM) glucose concentration, using Pan-Acetylation antibody. (c) IB detection of the transduced SFB-MDH2 acetylation level under 8-hour glucose starvation or not using the Pan-Acetylation antibody in GAS5 or Scramble control knocked down HEK293T cells. (d) SFB-MDH2 was transduced in empty vector control (EV), full-length GAS5 (FL) or GAS5 mutant (D2) overexpressed HEK293T cells. Acetylation of SFB-MDH2 was detected by IP-IB using indicated antibodies. (e-f) FLAG-CS (e) or FLAG-FH (f) was overexpressed in HEK293T and the acetylation of CS or FH was detected by IP-IB using indicated antibodies under indicated gradient glucose concentration for 8 hours.
