Extended Data Fig. 4: In vivo MRS quantitative measurement of brain ethanol metabolites and neurochemicals.
From: Brain ethanol metabolism by astrocytic ALDH2 drives the behavioural effects of ethanol intoxication
a, b, Representative peaks and Summary statistics of brain ALDH2 protein in cerebellum and liver from Aldh2Hep+/+ and Aldh2Hep-/-mice (n=4 mice/group).c, d, In vivo MRS measurement of cerebellar acetate and GABA contents after systemic ethanol (1-2 g/kg, i.p.) in Aldh2Gfap+/+ mice (n=5, 3 and 5 mice, respectively). e, Ethanol enhancement of cerebellar GABA, Glutamine, Glutamate and NAA in Aldh2 flox mice (n=9 mice/group). f, g, Statistic summaries of cerebellar ethanol by MRS after systemic administration of ethanol (1-2 g/kg, i.p.) in astrocytic ALDH2 deficient mice (f: n=3 mice/group; g: n=5 mice/group), hepatocytic ALDH2 deficient mice (Aldh2Hep+/+: n=4 mice and Aldh2Hep-/-: n=6 mice) and their wild type littermates mice. g, Bar graphs showing cerebellar ethanol concentrations after ethanol (2 g/kg, i.p.) by in vivo MRS measurement in Aldh2Hep+/+ (n=4) and Aldh2Hep-/- (n=4) mice. h, Bar graph showing the basal levels of GABA, Glutamine, Glutamate and NAA in cerebellum in Aldh2Gfap+/+ and Aldh2Gfap-/- mice (n=5 mice/group). i, Bar graph showing the basal levels of GABA, Glutamine, Glutamate and NAA in cerebellum in Aldh2Hep+/+ (n=4) and Aldh2Hep-/- (n=6) mice. Data are presented as mean±s.e.m. Groups were compared by unpaired two tailed student’s t test (b, e-i), or one-way analysis of variance (ANOVA) followed by Tukey’s test (c, d).
