Extended Data Fig. 4: Inhibition of MT1-MMP activity potentiates GDF15 functions.
From: Body weight regulation via MT1-MMP-mediated cleavage of GFRAL
(a) Change in body weight (%) in high fat diet-induced obese mice treated with control IgG or 3A2 after daily subcutaneous administration of recombinant GDF15 (10nmol/kg) for 6 days. (b-c) Cumulative food intake (b) and changes in food intake (%) (c) in mice with diet-induced obesity from (a). (d-e) Quantification of the percentage of GFRAL-positive cells that co-expressed cFOS in the area postrema of mice treated with control IgG or 3A2 (d) and the relative changes in cFOS +GFRAL+ cells (e) after receiving a single subcutaneous injection of recombinant GDF15 (10nmol/kg). Data are reported as average ± s.e.m. (n=6) P < 0.05; **P < 0.01; ***P < 0.001. two-sided unpaired t-test (c,e) or one-way ANOVA (a-b, d).
